肿瘤微创技术
MINIMALLY INVASIVE TECHNIQUES FOR TUMORS
脉冲电场疗法的原理主要基于脉冲电场诱导的细胞膜的电穿孔效应
电穿孔是细胞膜在外部电场作用下对离子和大分子的通透性增加的一种生物物理现象
在电场作用下，细胞膜的凝脂双分子层跨膜电位上升，脂质双分子发生偏转进行重排，形成稳定的亲水性微孔，允许生物分子（如离子、DNA）等透过
The principle of pulsed electric field therapy is mainly based on the electroporation phenomena of cell membranes induced by pulsed electric fields.
Electroporation is a biophysical phenomenon where the permeability of the cell membrane to ions and large molecules increases under the influence of an external electric field.
Under the electric fields, the transmembrane potential of the cell membrane increases, and the lipid bilayer of the membrane undergoes deformation and rearrangement, forming stable hydrophilic micropores that allow biomolecules (such as ions and DNA) to pass through.
微秒脉冲不可逆电穿孔技术，其通过采用双针电极向肿瘤病灶区施加80~120个脉冲宽度5~100 μs，场强为1000 V/cm以上的脉冲电场，诱导有效电场覆盖区域内肿瘤细胞发生不可逆电穿孔，引起细胞内大量电解质和ATP等跨膜转移，破坏细胞内外生理平衡，从而导致细胞死亡，达到消融肿瘤的目的
靶向细胞膜诱导不可逆电穿孔
诱导抗肿瘤免疫学响应
可用于组织消融、肿瘤消融、心脏房颤等
严重肌肉收缩，需要注射足量肌松剂，全麻条件进行
microsecond IRE utilizes a double-needle electrode to deliver 80-120 pulses with a pulse width of 5-100 μs and a field strength of over 1000 V/cm to the tumor lesion. This induces irreversible electroporation of tumor cells within the effective electric field coverage area, causing a massive transfer of intracellular electrolytes and ATP across the cell membrane, disrupting the physiological balance inside and outside the cell and then leading to cell death. Finely, we can achieve the purpose of ablating the tumor.
Induce irreversible electroporation on the cell membrane
activate the anti-tumor immune response
Can be use for tissue ablation、tumor ablation and atrial fibrillation treatment
severe muscle contraction requires adequate muscle relaxants and general anesthesia.
高强度的亚微秒或纳秒电脉冲（10~100 kV/cm）含有丰富的高频含量可作用到细胞内部结构，导致细胞一系列功能性的改变，包括DNA破坏、细胞骨架损伤、细胞膨胀、钙离子溢出、信号通路激活等，最终导致内源性或外源性细胞程序性死亡
靶向细胞膜诱导超穿孔效应；
靶向细胞内部细胞器膜，诱导内电处理效应；
诱导抗肿瘤免疫学响应；
可一定程度克服肿瘤异质性，提高电场敏感性；
细胞死亡阈值高、电刺激范围广、死亡区域小；
严重肌肉收缩，需要注射足量肌松剂，全麻条件进行；
High-intensity sub-microsecond or nanosecond electric pulses (10~100 kV/cm) with rich high-frequency content can act on internal structures of cells, leading to a series of functional changes, including DNA damage, cytoskeletal injury, cell swelling, calcium ion overflow, activation of signal pathways, etc., ultimately resulting in endogenous or exogenous programmed cell death.
Induce super-electroporation on the cell membrane.
Targeting the membranes of intracellular organelles to induce intracellular electromanipulation effect.
activate the anti-tumor immune response.
To a certain extent, can overcome the heterogeneity of tumors and increase the sensitivity to electric fields.
high lesion threshold, a wide range of electrical stimulation while a small area of cell death.
severe muscle contraction requires adequate muscle relaxants and general anesthesia.
通过宽频谱协同高压脉冲电场诱导细胞膜、细胞器膜多位点效应，引起细胞死亡，实现病灶组织的非热选择性消融
降低势垒，降低阈值，扩大消融范围
克服异质性，提高杀伤
诱导比单一脉冲序列更强免疫学响应
降低肌肉刺激和神经动作电位传播
无需注射肌肉松弛剂，可实现局麻条件下手术
using broad-spectrum synergistic high-voltage pulsed electric fields , multi-site effects on the cell membrane and organelle membranes can be induced, leading to cell death and achieving non-thermal selective ablation of diseased tissue
reduce the energy barrier, lower the threshold, and expand the ablation range.
overcome tumor heterogeneity, enhance the killing effect
stronger immune response compared to a single pulse sequence
reduce muscle stimulation and inhibit nerve action potential propagation
No muscle relaxants are required and can be performed under local anesthesia
